Paraffin Embedded Tissue

Diagnostic Cytogenetics, Inc. performs FISH on paraffin embedded tissue samples with a turnaround time of less than 3 days.

If you are interested in a probe that is not offered currently, please contact us and we will add the probe to our menu.

 

  • FFPE FISH Panels

    Panels can be customized upon request- please inquire for more details.

     

    Neoplastic FISH Panels

    Panel Probe Target Abnormality
    CLL panel ATM 11q22.3 deletion
    D12Z3 Trisomy 12
    D13S319/LAMP1 13q34 deletion/ monosomy 13
    TP53 17p13.1 deletion
    IGH/CCND1 t(11;14)(q13;q32)
    CML panel BCR/ABL1 t(9;22)(q34;q11.2)
    MM panel IGH/FGFR3 t(4;14)(p16;q32)
    D5S23/D5S721, CEP 9, CEP 15 Hyperdiploidy for chromosomes 5, 9, 15
    IGH/CCND1 t(11;14)(q13;q32)
    D13S319/LAMP1 13q34 deletion/ monosomy 13
    IGH Breakapart 14q32 rearrangements
    IGH/MAF t(14;16)(q32;q23)
    TP53 17p13.1 deletion
    NHL panel BCL6 3q27 rearrangements
    MYC BAP 8q24 rearrangements
    IGH/CCND1 t(11;14)(q13;q32)
    BIRC3/MALT1 t(11;18)(q21;q21)
    IGH/BCL2 t(14;18)(q32;q21)

     

    POC Aneuploidy FISH Panel

    The POC Aneuploidy Fluorescent In Situ Hybridization (FISH) panel detects the most common chromosome gains and losses seen prenatally.

    Probe Target Abnormality
    RB1 Trisomy 13
    16qhet Trisomy 16
    D18Z1 Trisomy 18
    D21S259 Trisomy 21
    BCR Trisomy 22
    DXZ1 Sex Chromosome Aneuploidy
    DYZ3 Sex Chromosome Aneuploidy

     

  • FFPE FISH Probes

    Probe Target Abnormality
    BCL6 3q27 rearrangements
    IGH/FGFR3 t(4;14)(p16;q32)
    D5S23/D5S721, CEP 9, CEP 15 Hyperdiploidy for chromosomes 5, 9, 15
    IGH/MYC/CEP8 t(8;14)(q24;q32), trisomy 8
    MYC Breakapart 8q24 rearrangements
    BCR/ABL1 t(9;22)(q34;q11.2)
    IGH/CCND1 t(11;14)(q13;q32)
    BIRC3/MALT1 t(11;18)(q21;q21)
    ATM 11q22.3 deletion
    D12Z3 Trisomy 12
    D13S319/LAMP1 13q34 deletion/ monosomy 13
    RB1 Trisomy 13
    IGH Breakapart 14q32 rearrangements
    IGH/MAF t(14;16)(q32;q23)
    IGH/BCL2 t(14;18)(q32;q21)
    PML/RARA t(15;17)(q24;q21)
    16qhet Trisomy 16
    TP53 17p13.1 deletion
    D21S259 Trisomy 21
    BCR Trisomy 22
    DXZ1 Sex chromosome aneuploidy
    DYZ3 Sex chromosome aneuploidy

     

    Methodology

    Fluorescence in-situ Hybridization (FISH) utilizes DNA probes that bind to a specific region of a chromosome. These probes produce bright colored signals that are examined on a fluorescence microscope.  Two hundred cells are scored.

     

  • Test Information

    Test Turnaround Time* CPT Codes**
    FISH on Paraffin Embedded Tissue  3 days or less 88377

    *All turnaround times are business days from specimen receipt to report issued.

    **Some codes may vary or be charged in multiple units, depending on patient-specific testing parameters.

     

  • Submitting a Sample

    Specimen Requirements

    Paraffin-Embedded Tissue Slides:  For each probe requested, submit a minimum of four slides of four-micron tissue sections from formalin-fixed, paraffin-embedded tissue blocks on positively-charged slides. The area of interest should be marked.  Pathology report and H&E slide of the tissue should be submitted.  All slides must be labeled with 2 unique identifiers that are identical to the accompanying request form.

     

    If possible, please provide slides of normal tissue of the same type to be used as a control.

     

    If paraffin blocks are submitted, there will be additional fees for processing.

     

    For more information, please see our Specimen Requirements and Shipping Information pages.

     

    Click here for our FFPE request form and insurance pre-authorization form.

Top